Sulfated mucopolysaccharide production by synovial sarcoma cells in vivo and in tissue culture.

and their growth medium demonstrate the ability of synovial sarcoma cells to synthesize and secrete various sulfated mucopolysaccharides. The sulfated species that is predominant inside the cell appears to be a heparin-related mucopolysaccharide. These findings clarify the histochemical observations that indicate the presence, in vivo, of mucopolysaccharide species resistant to degradation by hyaluronidase and chondroitinase ABC. The electrophoretic pattern of the sulfated species extracted from the synovial sarcoma cell cultures is quite different from that of the 35S-labeled mucopolysaccharides